OrangerV1, Main, Exploration, bibRecord, 002E18

Partial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat

Identifieur interne : 002E18 ( Main/Exploration ); précédent : 002E17; suivant : 002E19

Partial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat

Auteurs : L. Galipienso [Espagne] ; M. C. Vives [Espagne] ; P. Moreno [Espagne] ; R. G. Milne [Italie] ; L. Navarro [Espagne] ; J. Guerri [Espagne]

Source :

Archives of virology [ 0304-8608 ] ; 2001.

RBID : Pascal:02-0322488

Descripteurs français

Pascal (Inist)
- Virus, Citrus sinensis, Bourgeon, Propagation, Particule, Protéine végétale, RNA bicaténaire, Séquence nucléotide, Nucléotide, DNA complémentaire, Plante, Cultivar, Clone, Réplication, Citrus sinensis Poncirus trifoliata, Fortunella margarita.
Wicri :
- topic : Protéine végétale.

English descriptors

KwdEn :
- Bud, Citrus sinensis, Clone, Complementary DNA, Cultivar, Double stranded RNA, Nucleotide, Nucleotide sequence, Particle, Plant, Plant protein, Propagation, Replication, Virus.

Abstract

Citrus leaf blotch virus (CLBV) was purified from leaves of Nagami kumquat SRA-153 that showed bud union crease when propagated on Troyer citrange. Virions were filamentous particles (960 x 14 nm) containing a 42 kDa protein and a single-stranded RNA (ssRNA) of about 9,000 nt (Mr 3 x 10⁶). Infected tissue contained three species of double-stranded RNA (dsRNA) of Mr 6, 4.5 and 3.4 x 10⁶. The nucleotide sequence of several complementary DNA (cDNA) clones showed significant similarities with replication-related proteins from plant filamentous viruses in several genera. A digoxigenin-labelled probe from one of these cDNA clones hybridised in Northern blots with ssRNA from virions and with the three dsRNA species, suggesting that the ssRNA is the genomic RNA of the virus, the largest dsRNA is its replicative form, and the two smaller dsRNAs probably replicative forms of 5' co-terminal subgenomic RNAs. CLBV was also detected in several citrus cultivars from Spain and Japan including Navelina sweet orange field trees propagated on Troyer citrange showing bud union crease; however, no virus could be detected in other citrus trees with similar symptoms. This indicates that CLBV is not restricted to kumquat SRA-153, but its involvement in causing the bud union disorder remains unclear.

Affiliations:

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Le document en format XML

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<term>Nucleotide sequence</term>
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<front><div type="abstract" xml:lang="en">Citrus leaf blotch virus (CLBV) was purified from leaves of Nagami kumquat SRA-153 that showed bud union crease when propagated on Troyer citrange. Virions were filamentous particles (960 x 14 nm) containing a 42 kDa protein and a single-stranded RNA (ssRNA) of about 9,000 nt (Mr 3 x 10<sup>6</sup>
). Infected tissue contained three species of double-stranded RNA (dsRNA) of Mr 6, 4.5 and 3.4 x 10<sup>6</sup>
. The nucleotide sequence of several complementary DNA (cDNA) clones showed significant similarities with replication-related proteins from plant filamentous viruses in several genera. A digoxigenin-labelled probe from one of these cDNA clones hybridised in Northern blots with ssRNA from virions and with the three dsRNA species, suggesting that the ssRNA is the genomic RNA of the virus, the largest dsRNA is its replicative form, and the two smaller dsRNAs probably replicative forms of 5' co-terminal subgenomic RNAs. CLBV was also detected in several citrus cultivars from Spain and Japan including Navelina sweet orange field trees propagated on Troyer citrange showing bud union crease; however, no virus could be detected in other citrus trees with similar symptoms. This indicates that CLBV is not restricted to kumquat SRA-153, but its involvement in causing the bud union disorder remains unclear.</div>
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Partial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat

Partial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri